产品目录

分子量：Approximately 9.3kDa,a single non-glycosylated polypeptide chain containing 82 amino acids.
储存条件：冷冻（-20℃）

概述

Murine CCL12 is belonging to the CC chemokine family.It shows 66%amino acid sequence identity to human MCP-1.CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils.At high concentrations,it will also chemoattract eosinophils.CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2,but can not induce a calcium flux in HEK-293 cells transfected with CCR1,CCR3,or CCR5.That shows the functional receptor of CCL12 is CCR2.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50ng/ml.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素	Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG

相关搜索：重组小鼠MCP-5/CCL12，MCP-5/CCL12, Murine